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Bachem substrate ac-devd-afc #4027914
Substrate Ac Devd Afc #4027914, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
substrate ac-devd-afc #4027914 - by Bioz Stars, 2026-10
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Article Title: Comparative study of the differential cell death protecting effect of various ROS scavengers.
Article Snippet: Biological Chemistry ‘Just Accepted’ Papers are papers published online, in advance of appearing in the print journal.. They have been peer-reviewed, accepted and are online published in manuscript form, but have not been copy edited, typeset, or proofread.. Copy editing may lead to small differences between the Just Accepted version and the final version.

Article Title: Intracellular cathepsin C levels determine sensitivity of cells to leucyl-leucine methyl ester-triggered apoptosis.
Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/FEBS.15326 This article is protected by copyright.. All rights reserved PROF. BORIS TURK (Orcid ID : 0000-0002-9007-5764) Received Date : 04-Jul-2019 Revised Date : 06-Feb-2020 Accepted Date : 01-Apr-2020 Color : Fig 1-9

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

Article Title: Menadione-induced apoptosis in U937 cells involves Bid cleavage and stefin B degradation.
Article Snippet: Funding information Javna Agencija za Raziskovalno Dejavnost RS, Grant/Award Number: P1‐0140 Abstract Earlier studies showed that the oxidant menadione (MD) induces apoptosis in certain cells and also has anticancer effects.. Most of these studies emphasized the role of the mitochondria in this process.. However, the engagement of other organelles is less known.

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: DEVDase activity and FACS analysis 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

Article Title: Resveratrol Modulates Diabetes-Induced Neuropathic Pain, Apoptosis, and Oxidative Neurotoxicity in Mice Through TRPV4 Channel Inhibition.
Article Snippet: Active caspases -3, -8, and -9 predominantly cleaved fluorogenic substrates (Ac-DEVD-AMC, Ac-IETD-AFC, and Ac-LEHD-AFC) (Bachem AG., Bubendorf, Switzerland) to the AMC and AFCs following incubation with apoptotic cell lysates in black plates, respectively.

FACS:


Article Title: Comparative study of the differential cell death protecting effect of various ROS scavengers.
Article Snippet: Biological Chemistry ‘Just Accepted’ Papers are papers published online, in advance of appearing in the print journal.. They have been peer-reviewed, accepted and are online published in manuscript form, but have not been copy edited, typeset, or proofread.. Copy editing may lead to small differences between the Just Accepted version and the final version.

Article Title: Intracellular cathepsin C levels determine sensitivity of cells to leucyl-leucine methyl ester-triggered apoptosis.
Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/FEBS.15326 This article is protected by copyright.. All rights reserved PROF. BORIS TURK (Orcid ID : 0000-0002-9007-5764) Received Date : 04-Jul-2019 Revised Date : 06-Feb-2020 Accepted Date : 01-Apr-2020 Color : Fig 1-9

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

Article Title: Menadione-induced apoptosis in U937 cells involves Bid cleavage and stefin B degradation.
Article Snippet: Funding information Javna Agencija za Raziskovalno Dejavnost RS, Grant/Award Number: P1‐0140 Abstract Earlier studies showed that the oxidant menadione (MD) induces apoptosis in certain cells and also has anticancer effects.. Most of these studies emphasized the role of the mitochondria in this process.. However, the engagement of other organelles is less known.

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: DEVDase activity and FACS analysis 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

Article Title: Resveratrol Modulates Diabetes-Induced Neuropathic Pain, Apoptosis, and Oxidative Neurotoxicity in Mice Through TRPV4 Channel Inhibition.
Article Snippet: Active caspases -3, -8, and -9 predominantly cleaved fluorogenic substrates (Ac-DEVD-AMC, Ac-IETD-AFC, and Ac-LEHD-AFC) (Bachem AG., Bubendorf, Switzerland) to the AMC and AFCs following incubation with apoptotic cell lysates in black plates, respectively.



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Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage <t>(Ac-DEVD-AFC)</t> by activated caspase-3 in two independent experiments.
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Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage <t>(Ac-DEVD-AFC)</t> by activated caspase-3 in two independent experiments.
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Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage <t>(Ac-DEVD-AFC)</t> by activated caspase-3 in two independent experiments.
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Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage <t>(Ac-DEVD-AFC)</t> by activated caspase-3 in two independent experiments.
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Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage <t>(Ac-DEVD-AFC)</t> by activated caspase-3 in two independent experiments.
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Bachem substrate ac-devd-afc #4027914
Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage <t>(Ac-DEVD-AFC)</t> by activated caspase-3 in two independent experiments.
Substrate Ac Devd Afc #4027914, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage <t>(Ac-DEVD-AFC)</t> by activated caspase-3 in two independent experiments.
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Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage (Ac-DEVD-AFC) by activated caspase-3 in two independent experiments.

Journal: Neoplasia (New York, N.Y.)

Article Title: Rational payload selection enables high antitumoral efficacy of an anti-EGFR antibody-drug conjugate against ovarian tumors

doi: 10.1016/j.neo.2026.101295

Figure Lengend Snippet: Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage (Ac-DEVD-AFC) by activated caspase-3 in two independent experiments.

Article Snippet: The payloads used for antibodies conjugation, MC-GGFG-DXD (DXd, catalog reference: HY-13631E), MC-Val-Cit-PAB-MMAF (Vc-MMAF, catalog reference: HY-112786) and McMMAF (catalog reference: HY-15578), the belantamab mafodotin ADC (catalog reference: HY-P3239), as well as the Ac-DEVD-AFC (catalog reference: HY-P1005) substrate for caspase-3 activity detection, were purchased from MedChem Express (Princeton, NJ, USA).

Techniques: Flow Cytometry, Apoptosis Assay, Western Blot, Control, Molecular Weight, Activity Assay, Fluorescence